HPLC Analysis of Fatty Acids and Triterpenoids in Fruit of Hawthorn (Crataegus azarolus) in Iraqi Kurdistan Region

摘要

Fatty acids and triterpenoids content of three types of hawthorn fruit {Crataegus azarolus) which grows in Iraqi Kurdistan region (season 2011) including Hawraman, Qaradax and house garden, were determined by RP-HPLC technique. The percentages of oils were found to be 12, 10 and 14 %, respectively. HPLC analysis of these oils show contain palmatic acid, palmitoleic (ω 7) acid and high levels of a-linoleic acid about [37.202, 31.402 and 33,816 %], Imoleic acid [9.686, 21.556 and 19,135 %], respectively. Divert compounds of triterpenoids composition were also analyzed by HPLC technique in all of them mentioned four triterpenoids compound were detected oleanolic acid, ursolic acid, α-amyrin, β-amyrin and a high ratio (choline, acetylcholine) have also been detected. The ratio of acetylcholine is about 53.800, 24.6144 and 30.8198 % in each types, respectively and a high level of choline detected in Qaradax hawthorn fruits is about 39.2849 % and in house garden about 35.4523 %.