Development and Validation of a New HPTLC Method for Determination of Protopine in Accelerated Solvent Extract of Fumaria vaillantii Loisel

摘要

A new, simple and accurate HPTLC method is developed using ICH guidelines for the determination of protopine in metftanolic whole plant extract of Fumaria vaillantii Loisel Accelerated solvent extraction CASE) as an alternative to convention techniques was also explored for the rapid extraction. Chromatographic separation was performed on silica gel 60 F_(254) HPTLC plates in the mobile phase of methyl ethyl ketonerethyl acetate: formic acid (5:3:2, v/v/v) and the plates were scanned deasitometricaDy at 2°3 nm (Ann of protopine). The amount of protopine was found in range L29-1.41 and 1.47-1.54 mg/g in Soxblet and-ASB 80 °C extracted plant sample; respectively. The HPTLC method was validated in terms of sensitivity, accuracy, precision and reprodacibiluy. Accelerated solvent extraction method has higher extraction efficiency in less time as compared to conventional Soxhlet extraction. Rapid and reproducible method is useful for routine analysis of protopine and quality control of F. vaillantii.