Amperometric Glucose Sensor Based on The Entrapment of Glucose Oxidase in Electrochemically Synthesized Pyrrole/ N,N-Dimethylaminopyrrole(Py/DMAPy)Copolymer Film

摘要

Glucose oxidase(GOx)was entrapped on the films obtained by electrochemical copolymerization of pyrrole(Py)and N,N-dimethyl-aminopyrrole(DMAPy),to fabricate iGOx-immobilized electrodes for amperometric sensing of glucose.Polymeric film was characterized with cyclic voltammetry(CV)and Fourier transform infrared(FTIR)spectroscopy.The amperometric response of the enzyme electrode was measured at+0.70V vs.Ag/AgCl,which was due to the electrooxidation of enzyma-tically,produced H2O2.The effects of the composition of copolymer,pH,temperature and substrate concentration on the response of the enzyme electrode were investigated.The optimum pH was found to be 7.4 at 25 °C and response time of enzyme electrode was determined as 45 s.The upper limit of the linear working portion was obtained as 10 mM glucose concentration with a detection limit of(5.0 X 10~(-6)M)with the calculated value of apparent Michaelis-Menten constant(KMapp)was 3.70 mM.The activation energy of this immobilized enzyme system were found to be 30.25 kJ mol~(-1).