Estimation of Acipimox in Bulk Drug and Capsules by RP-HPLC Method

摘要

A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of acipimox in bulk drag and pharmaceutical dosage forms. The quantification was carried out on Luna C_(18) column in isocratic mode, with mobile phase consisting of 0.1 % v/v phosphoric acid in water and acetonitrile in the ratio of 95:5 [ v/v ]. The mobile phase was pumped at a rate of 1.0 mL/min and the detection was carried out at 229 nm and the linearity was found to be in the range of 20-300 μg/mL. The regression equation was found to be Y = 33672x + 6367.2 with correlation coefficient [r~2] of 0.9998. The percentage recovery values were found to be in the range of 99.96-100.07 %. Validation of the proposed method has also been done.