Studies on the Genetic Variability of Three Fish Species (Cyprinus carpio specularis, Cyprinus carpio communis and Oncorhynchus mykiss) Collected from Kashmir (India) Using Random Amplified Polymorphic DNA (RAPD) Technique

摘要

The present study evaluated the patterns of morphometric and genetic variation using RAPD-PCR techniques for the first time on two species of Cyprinus and single species of Oncorhynchus mykiss from Srinagar, Kashmir, India. The Random Amplified Polymorphic DNA (RAPD) analysis was used to estimate the genetic relationships and diversity of three species of local fishes (Family Cyprinidae). A large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the present study, genetic variability of three local fish species (Cyprinus carpio specularis, Cyprinus carpio communis and Oncorhynchus mykiss) collected from Dal Lake and local Trout Fish Farm (Harwan, Srinagar, India) was analyzed using the RAPD-PCR technique. Amplified DNA fragments with monomorphic profile were not found in the studied species, except for Cyprinus carpio specularis and Cyprinus carpio communis which presented monomorphic bands as both belong to the same genus. All speciesshowed high levels of genetic variability among individuals. Cyprinus carpio communis and Cyprinus carpio specularis are more similar in comparison to the other Oncorhynchus mykiss based on morphological approach.