Lymph node derived Theileria lestoquardi vaccine in sheep.

摘要

Theileria lestoquardi causes malignant theileriosis in sheep and goats. We isolated theileria infected cells from prescapular lymph node of an adult ewe. By PCR we confirmed that ovine lymphocytes were infected by Theileria lestoquardi. DNA was extracted for primers RLB-F2 for Theileria and RLB-R2 for Babesia amplified V4 hypervariable region of 18S rRNA genes. Then 6 animals were inoculated subcutaneously with 5 x 10s cells infected by T. lestoquardi from 5th culture passage. Controls were 4 sheep injected with normal lymphocytes. Intradermal skin test were done 2 months after infection with freeze-dried Theileria reconstituted antigen by 0.1ml vaccine intradermal injection in infected and controls. Skin thickness was measured by caliper at 72h. Wefound found no clinical effects. However, in infected sheep we found 1mm thickening by 72h but none in controls. Findings suggested non-attenuated cells did not induce side effects and skin reaction which may have boosted cellular immunity.