Male Accessory Gland Derived Fators Can Stimulate Oogenesis and Enhance Oviposition in Helicoverpa armigera (Lepidoptera: Noctuidae)

摘要

In Helicoverpa armigera, female moths began to lay eggs on the third day after emergence. Mating stimulated earlier egg matu- ration/oogenesis (P = 0.002) and oviposition (P ? 0.01). We estab- lished a suitable bioassay model for the influence of male accessory glands (MAG) on the physiology of virgin females: Crude extracts of MAG (2- to 3-day-old) were injected into 2-day-old vir- gin females, and the injected females were dissected 20 h after mating. It was shown that crude extracts of MAG stimulated ear- lier egg maturation (P < 0.001) and oviposition (the oviposition ratio was more than 2 times the ratio of the control). Proteina- ceous components in crude extracts purified by fractionation and sub-fractionation in reverse phase high performance liquid chro- matography also stimulated earlier egg maturation (P < 0.01) and ovipositon (more than 2 times the ratio of the control), and we called them the oogenesis and ovipostion factors (OOSF). With SDS-PAGE, the molecular mass of the bands from OOSF was es- timated to be between 55-66 KD. Arch. Insect Biochem.