首页> 外文期刊>Archives of Insect Biochemistry and Physiology >Molecular characterization of the ebony gene from the American cockroach, Periplaneta americana
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Molecular characterization of the ebony gene from the American cockroach, Periplaneta americana

机译:美国蟑螂美洲黑檀乌木基因的分子表征

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Biogenic amines are an important class of primary messengers in the central (CNS) and peripheral nervous systems and in peripheral organs. These substances regulate and modulate many physiological and behavioral processes. Various inactivation mechanisms for these substances exist to terminate biogenic amine-mediated signal transduction. In vertebrates, the enzymes monoamine oxidase and/or catechol-O-methyl-transferase are involved in these processes. In insects, however, in which both enzymes are low in abundance or absent, biogenic amines are inactivated mainly by N-acetylation or O-sulphation. In Drosophila, beta-alanyl conjugation mediated by the Ebony protein has recently been shown to be a novel and alternative pathway for biogenic amine inactivation. Here, we report the cloning of ebony cDNA (Peaebony) from a brain-specific cDNA library of the cockroach Periplaneta americana. The open reading frame encodes a protein of 860 amino acid residues (PeaEbony). The PeaEbony polypeptide shares homology to Ebony sequences from Anopheles gambiae, Apis mellifera, and Drosophila melanogaster. In addition, PeaEbony exhibits sequence similarity to a family of microbial non-ribosomal peptide synthetases. The mRNA encoding PeaEbony is highly expressed in the cockroach brain and to a lesser extent in the salivary glands. PeaEbony is, therefore, probably involved in the inactivation of various biogenic amines through beta-alanyl conjugation in the cockroach CNS. Since the salivary glands in Periplaneta are innervated by dopaminergic and serotonergic neurons, PeaEbony probably also biochemically modifies dopamine and serotonin in these acinar glands.
机译:生物胺是中枢(CNS)和周围神经系统以及周围器官中重要的一类主要信使。这些物质调节和调节许多生理和行为过程。存在用于这些物质的各种灭活机制,以终止生物胺介导的信号转导。在脊椎动物中,单胺氧化酶和/或儿茶酚-O-甲基转移酶参与了这些过程。然而,在昆虫中,两种酶的丰度都低或不存在,生物胺主要通过N-乙酰化或O-硫酸化而失活。在果蝇中,最近已证明,由乌木蛋白介导的β-丙氨酰偶联是生物胺失活的一种新颖的替代途径。在这里,我们报道了从美洲蟑螂的大脑特异性cDNA文库中克隆出乌木cDNA(牡丹)。开放阅读框编码860个氨基酸残基的蛋白质(PeaEbony)。 PeaEbony多肽与冈比亚按蚊,蜜蜂和黑腹果蝇的乌木序列具有同源性。另外,PeaEbony与微生物非核糖体肽合成酶家族表现出序列相似性。编码PeaEbony的mRNA在蟑螂脑中高表达,而在唾液腺中表达较少。因此,PeaEbony可能通过蟑螂CNS中的β-丙氨酰结合而使各种生物胺失活。由于腹膜周围的唾液腺受多巴胺能和5-羟色胺能神经元的支配,因此PeaEbony可能还可以生化地修饰这些腺泡中的多巴胺和5-羟色胺。

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