Relationship between calcium mobilization and platelet alpha- and delta-granule secretion. A role for TRPC6 in thrombin-evoked delta-granule exocytosis

摘要

Changes in cytosolic Ca2+ concentration ([Ca2+](c)) regulate granule secretion in different cell types. Thrombin activates PAR1 and PAR4 receptors and promotes release of Ca2+ from distinct intracellular stores, which, in turn, activates store-operated Ca2+ entry (SOCE). A crucial step during platelet function is the release of physiological agonists stored in secretory granules to the extracellular compartment during activation. We aim to study the role of Ca2+ mobilization from the extracellular compartment or from different intracellular stores in platelet granule secretion. By using flow cytometry, we have found that alpha- and delta-granules are secreted in thrombin-stimulated platelets in the absence of extracellular Ca2+, and in a concentration-dependent manner. Our findings show that thrombin-stimulated granule secretion depends on Ca2+ mobilization from intracellular stores. Analysis of the kinetics of granule secretion reveals that platelet stimulation with thrombin results in rapid release of alpha-granules which precedes the secretion of delta-granules. Incubation of platelets with a specific antibody, which recognizes the extracellular amino acid sequence 573-586 of TRPC6, inhibited thrombin-evoked delta-granule exocytosis. Our results indicate that the mechanisms underlying thrombin-induced alpha- and delta-granule secretion show differences in dependency on Ca2+ mobilization. (C) 2015 Elsevier Inc. All rights reserved.