Artificial Enzyme Mimics for Catalysis and Double Natural Enzyme Co-immobilization

摘要

This work presents a new chemiluminescent (CL) probe array assay. The new type CL probe array is based on enzyme mimics of Co_3O_4–SiO_2 mesoporous nanocomposite material, which not only have an excellent catalytic effect on the luminol–H_2O_2 CL reaction in an alkaline medium but also can be used for the immobilization of enzymes. The linear range of the lactose concentration is 3.0×10~(?7) to 1.0×10~(?5) g mL~(?1) and the detection limit is 6.9×10~(?8) g mL~(?1). β-Galactosidase and glucose oxidase were selected as a model for enzyme assays to demonstrate the applicability of Co_3O_4–SiO_2 mesoporous nanocomposite material in multienzyme immobilization. The novel bifunctional CL probe array has been successfully applied to the determination of lactose in milk.