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Highly sensitive detection of caspase-3 activity based on peptide-modified organic electrochemical transistor biosensors

机译:caspase-3活动的高度敏感的检测基于peptide-modified有机电化学晶体管生物传感器

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Caspase-3 is an important proteolytic enzyme that cleaves several key substrates in apoptotic processes, resulting in DNA fragmentation, the degradation of nuclear proteins, and the formation of apoptotic bodies. However, it is challenging to detect caspase-3 due to its low expression levels in cells. In this work, organic electrochemical transistors (OECTs) are used in the detection of caspase-3 for the first time. A self-assembled monolayer of the peptide is bonded to the Au gate electrode (GE) of an OECT via gold-sulphur bonds. It is found that the transfer curve of the transistor shifts to a lower gate voltage due to the modulation of the surface potential of the GE by the peptides. Then, the device is used in the detection of caspase-3 in aqueous solutions and shows a detection limit of 0.1 pM. Due to its high sensitivity, the device can detect caspase-3 in induced apoptotic HeLa cells. The system is low-cost, conveniently used and applicable for biological and medical monitoring where caspase-3 detection and quantification are required.
机译:Caspase-3是一个重要的蛋白水解酶在凋亡劈开几个关键基质过程,导致DNA碎片,退化核的蛋白质,凋亡体的形成。具有挑战性的检测caspase-3由于其低细胞中的表达水平。电化学晶体管(OECTs)中使用首次caspase-3的检测。自组装单层的肽键非盟栅电极(GE) OECT通过gold-sulphur债券。晶体管曲线转移到一个较低的门由于表面的调制电压潜在的通用电气的肽。设备用于caspase-3的检测水解决方案,显示了一个检测极限0.1点。可以在诱导凋亡检测caspase-3海拉细胞。适用于生物和医学caspase-3检测和监控量化是必需的。

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