Temperature-dependent pharmacokinetics and tissue distribution of oxolinic acid in sea bass, Dicentrarchus labrax L., after a single intravascular injection

摘要

The pharmacokinetics and tissue distribution of oxolinic acid following an intravascular administration (15 mg kg(-1) fish) were determined in sea bass, Dicentrarchus labrax L. (110 g), at 13 degreesC and 22 degreesC water temperature. The kinetic profile of the drug was found to be temperature dependent, with increased temperature having a greater effect on distribution after equilibrium and the elimination phase than on the distribution process. The distibution half-life of oxolinic acid was 1.15 and 2.76 h at 22degreesC and 13degreesC respectively, whereas the elimination half-life of the drug was 55 h at 22degreesC and 315 h at 13 degreesC. The values of the apparent volume of distribution (1.44 Lkg(-1) at 22degreesC and 3.31 Lkg(-1) at 13 degreesC) and the volume of distribution at steady state (5.2 and 14.7 L kg(-1) at the high and low temperature respectively) were considerably different between the two tested temperatures. The total body clearance of the antibiotic was found to be low (1.47 L kg(-1) day(-1) at 22degreesC and 0.80 L kg(-1) day(-1) at 13 degreesC). Lower rates of elimination were found for the liver compared with muscle, the difference increasing with increasing temperature, while elimination rates from the serum were higher than those of other tissues, especially at the high temperature.