Effects of probiotics (Pseudomonas synxantha and Pseudomonas aeruginosa) on the growth, survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye, 1896)

摘要

The effects of two selected probiotics (Pseudomonas synxantha and Pseudomonas aeruginosa) on the specific growth rate (SGR), survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus) were examined for 84 days. Pseudomonas synxantha and P. aeruginosa were either applied into the rearing medium or supplemented in the formulated feed at a concentration of 10e colony forming unit (CFU) mLp# for each probiotic. The results showed that applications of the probiotics resulted in no significant difference (P>0.05) in the SGR and survival of the prawns, but significantly decreased the food conversion ratios (P<0.05) compared with the control where no probiotics were applied. The supplement of the probiotics with the formulated feed enhanced the probiotic numbers in the intestines of the prawns and low bacteria numbers in the haemolymph. The prawns were significantly (P<0.05) healthier, as indicated by the immune response obtained when both probiotics were given in combination compared with when individual probiotics were used. When applied singly, P. aeruginosa resulted in a higher health status of the prawns compared with P. synxantha. Overall, equal combinations (10e CFU mLp#) and volumes of P. synxantha and P. aeruginosa used as a supplement in the formulated feed resulted in an improvement in the health of the juvenile western king prawns.