Effects of incubation temperature on development and yolk sac conversion efficiencies of spotted wolffish (Anarhichas minor Olafsen) embryos until hatch.

摘要

Two egg batches of spotted wolffish, Anarhichas minor Olafsen, were incubated at 4, 6 and 8 degrees C. Embryo samples were fixed and compared on each 100th daydegree until hatching (up to 1000 daydegrees). Embryos, yolk sacs and chorions were dissected and the sizes, wet and dry weights were recorded separately. Comparisons of gross morphologies and measured parameters showed increasing and generally significant differences with time between the incubation temperatures. Lower temperatures produced longer and more differentiated larvae at hatch with a smaller yolk sac. Even though some unexpected deviations were registered among batches and experimental groups, it was clear that temperature affected embryo survival and time of hatching. Overall survival was best at 6 degrees C, in agreement with results from earlier studies. Yolk conversion efficiencies measured around the hatching point were generally high, ranging from 60% to 78%, varied between the two batches and probably reflected the developmental variations between embryos and larvae at the respective ages (daydegrees). The hatching process was apparently an energy-demanding period; yolk conversion efficiencies of unhatched embryos of similar age at each temperature were always higher. Temperature is one environmental factor that can be manipulated in hatcheries to induce hatching of viable larvae at an optimal stage of differentiation with respect to first-feeding success and early survival..