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Negative Reagent Controls in Diagnostic Immunohistochemistry: Do We Need Them? An Evidence-based Recommendation for Laboratories Throughout the World

机译:诊断性免疫组织化学中的阴性试剂对照:我们需要它们吗?全世界实验室的循证建议

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摘要

During the development and application of immunohistochemical techniques to diagnostic pathology, controls have played an important role in ensuring that the appropriate result has been obtained. The positive control is a tissue or collection of cells that contains the target protein (antigen) and confirms that the primary antibody and detection system have worked properly. The negative control, used primarily for primary antibody validation, is a specimen that does not contain the target protein and, as a result, should show no immunoreactivity. The negative reagent control (NRC) is an additional tissue section (frozen or paraffin) or cytology smear prepared from the patient specimen that is treated identically to that of the test slide(s) except for the omission of the primary antibody. Its primary purpose is to identify nonspecific reactivity due to factors or components other than the primary antibody.
机译:在免疫组织化学技术的发展和诊断病理学的应用中,对照在确保获得适当结果方面起着重要作用。阳性对照是包含靶蛋白(抗原)并确认一抗和检测系统正常工作的组织或细胞集合。阴性对照主要用于一抗验证,是一个不含靶蛋白的标本,因此不显示免疫反应性。阴性试剂对照(NRC)是由患者标本制备的其他组织切片(冷冻或石蜡)或细胞学涂片,除省略了第一抗体外,均与测试载玻片进行了相同的处理。其主要目的是鉴定由于非第一抗体的因素或成分而引起的非特异性反应性。

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