Bovine serum albumin: Survival and osmolarity effect in bovine spermatozoa stored above freezing point

摘要

Liquid nitrogen preservation in remote farms is a limitation. The goal of this study was to determine optimum temperature above freezing point for bovine spermatozoa preservation using bovine serum albumin (BSA) as a supplementation. Pooled semen sample from three ejaculates was subjected to various BSA concentration (1, 4, 8 and 12mgml -1), before incubation in different above freezing point temperatures (4, 25 and 37°C). Viability assessment was carried out against time from day 0 (fresh sample) until all spermatozoa become nonviable. Optimal condition for bovine spermatozoa storage was at 4°C with 1mgml -1 BSA for almost 7days. BSA improved bovine spermatozoa viability declining rate to 44.28% at day 4 and 57.59% at day 7 compared to control, with 80.54% and 98.57% at day 4 and 7 respectively. Increase in BSA concentration did not improve sperm viability. Our results also confirmed that there was a strong negative correlation between media osmolarity and bovine spermatozoa survival rate with r=0.885, P0.0001. Bovine serum albumin helps to improve survival rate of bovine spermatozoa stored above freezing point.