MicroRNA-146 regulates the inflammatory cytokines expression in vascular endothelial cells during sepsis

摘要

Aims: The purpose of this study was to investigate the functional role of microRNA (miR)-146 in sepsis, as well as the underlying mechanism. Methods: Human vascular endothelial cell line EA. hy926 cells were treated with lipopolysaccharide (LPS) and/or transfected withmiR-146 mimics, inhibitor, and their corresponding controls. Expression of miR-146 was then analyzed after treatment and/or transfection, as well as the expression of inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, intercellular adhesion molecule(ICAM)-1, and E-selectin, and nuclear factor kappa B (NF-κB) binding activity. Results: The results showed that the expression of miR-146 was significantly downregulated by LPS stimulation compared to the control group (P P P P Conclusions: MiR-146 may play an important role in the pathogenesis and development of sepsis by suppressing the expression of inflammatory cytokines.