The effect of bone marrow mesenchymal stem cells on highly metastatic MHCC97-H hepatocellular carcinoma cells following OPN and TGF beta 1 gene silencing

摘要

The metastatic behavior of hepatocellular carcinoma (HCC) is one of the key factors that leads to poor prognosis. The aim of the current study was to determine the changes in metastasis and the proliferation potential of bone marrow mesenchymal stem cells (BMSCs) in high metastatic potential hepatocellular carcinoma (MHCC97-H) following gene silencing. The osteopontin (OPN) and transforming growth factor-beta (TGF beta (1)) genes, which are associated with metastasis and tumor proliferation, were silenced in MHCC97-H cells. Transwell assays were used to evaluate the migration of MHCC97-H cells in vitro. Additionally, a murine model of MHCC97-H lung metastasis was established. Following OPN and TGF beta (1) silencing, the migration of MHCC97-H cells was significantly reduced following BMSC intervention (P (1)-silenced animals, and their integrated optical density (IOD) value was significantly lower compared with controls (P (v)beta (3) expression in the OPN- and TGF beta (1)-silenced groups compared with controls (P>0.05). The metastasis and proliferation potential of MHCC97-H following BMSC intervention were significantly reduced in vitro and in vivo, especially in the TGF beta (1)-silenced group. The decrease in the metastatic potential in gene-silenced MHCC97-H cells was not associated with integrin alpha (v)beta (3) expression. Therefore, OPN and TGF beta (1) may be potential targets for HCC treatment, and TGF beta (1) may have a higher therapeutic potential for BMSC intervention.