Inhibitory effects of cytosine deaminase gene-transfected bone marrow mesenchymal stem cells on glioma cell proliferation

摘要

Bone marrow mesenchymal stem cells were isolated from C57BL mice,transfected with the cytosine deaminase(CD) gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes.C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells.C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma.Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days.MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma,as well as encased and reduced the volume of the glioma.CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.