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Role of calcium binding in protein structural changes and phospholipid-protein interactions studied by capillary isoelectric focusing with whole column imaging detection

机译:钙等离子在蛋白质结构变化和磷脂-蛋白质相互作用中的作用通过毛细管等电聚焦与全柱成像检测研究

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A variety of biochemical and physical properties of proteins are regulated by calcium ion (Ca~(2+)) binding with varying specificity and affinity.Calcium ion binding can adjust the phospholipid-protein interactions through changing the properties of phospholipid membrane.As an attractive detection technique,whole column imaging detection (WCID) coupled to capillary isoelectric focusing (cIEF) displays several advantages in the study of protein-ligand and protein-protein interactions,including fast and high-efficient separation,high resolution,and simple operation.In this study,a cIEF-WCID method was evaluated for studying the effect of Ca~(2+) binding on protein structural changes and phospholipid-protein interactions.Four proteins with different isoelectirc point (pI),trypsin inhibitor (pI=4.5),beta-lactoglobulin B (pI= 5.2),phosphorylase b (pI=6.3),and trypsinogen (pI= 9.3),were used for this purpose.The targeted proteins exhibited altered cIEF profiles due to protein conformation changes resulting from the Ca~(2+) binding.The study showed that Ca~(2+) can be buried in the phospholipid membrane,modify the membrane property,and change the phospholipid-protein interactions.The utility of the cIEF-WCID technique demonstrates that the calcium binding plays a crucial role in the protein structural changes and the phospholipid-protein interactions,and elucidates the possible mechanisms involved in calcium-protein binding and calcium bound phospholipid-protein interactions.
机译:钙离子(Ca〜(2+))结合具有不同的特异性和亲和力,可调节蛋白质的多种生化和物理特性。钙离子结合可通过改变磷脂膜的性质来调节磷脂-蛋白质相互作用。检测技术,全柱成像检测(WCID)与毛细管等电聚焦(cIEF)耦合在蛋白质-配体和蛋白质-蛋白质相互作用的研究中显示出多个优势,包括快速和高效的分离,高分辨率和简单的操作。本研究采用cIEF-WCID方法研究Ca〜(2+)结合对蛋白质结构变化和磷脂-蛋白质相互作用的影响。四种具有等电点(pI),胰蛋白酶抑制剂(pI = 4.5)的蛋白质,为此目的,使用了β-乳球蛋白B(pI = 5.2),磷酸化酶b(pI = 6.3)和胰蛋白酶原(pI = 9.3)。由于蛋白质构象ch,目标蛋白质显示出改变的cIEF特性研究表明,Ca〜(2+)可以被埋在磷脂膜中,改变膜的性质,并改变磷脂-蛋白质的相互作用。cIEF-WCID的效用技术表明钙结合在蛋白质结构变化和磷脂-蛋白质相互作用中起着至关重要的作用,并阐明了钙-蛋白质结合和钙结合的磷脂-蛋白质相互作用的可能机制。

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