Quick and Sensitive Determination of Fluoroquinolones by Capillary Electrophoresis_Potential Gradient Detection

摘要

A rapid, capillary electrophoresis-potential gradient detection method was developed for determining three fluoroquinolones: rufloxacin, enoxacin, and moxifloxacin. The fluoroquinolones were baseline separated within 3.5 min with background electrolyte composed of 50 mM acetic acid and 6 mM potassium hydro_xide at pH 3.7. For the first time, electrokinetic injection was applied in capillary electrophoretic separation of fluoroquinolones, and an average of ca. 10-fold improvement in detection limits was observed. The method was applied to a fortified chicken tissue sample with detection limits (S/N = 3) of 22-24 ngg-', suggesting the potential of the method for determining fluoroquinolone residues in real samples.