Rapid Determination of Minocyclinein Pharmaceutical Formulations and HumanUrine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene SulfonateLuminescence System

摘要

A new luminescence method based on the minocycline (MNC)-europium (Eu~(3+))-sodium dodecylbenzene sulfonate (SDBS) system was devel-oped for the determination of MNC. SDBS formed a ternary complex withMNC-Eu~(3+)and significantly enhanced the luminescence intensity of Eu~(3+). Theenhanced luminescence intensity showed a good linear relationship with theconcentration of MNC over the range of 4.0 × 10~(-7)～1.0 × 10~(-5)mol L~(-1)with adetection limit of 2.0 × 10~(-8)mol L~(-1).This method is rapid and sensitive, andhas been successfully applied for the determination of MNC in capsules andhuman urine/serum samples. The luminescence mechanism is also studied.