A DNA Nanomachine Based on a Duplex-Triplex Transition

摘要

Herein we report the construction of a DNA nanomachine whose motion is triggered by changes in the solution pH value.Reversible formation and dissociation of a DNA triplex containing C~+G-C triplets take place when the solution pH value changes between pH5.0 and 8.0.This process constitutes the working principle of our DNA machine.We demonstrated the operation of the DNA machine by using fluorescence resonance energy transfer(FRET)techniques,and confirmed its structural integrity by native polyacrylamide gel electrophoresis(PAGE).