Bisdemethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-kappa B, MMP-2 and-9 Pathways

摘要

Background/Aim: Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and antimetastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells. Materials and Methods: Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro. Results: BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERKJ/2), E-cadherin and nuclear factor-kappa B (NF-kappa B) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-kappa B when compared to controls. Conclusion: BDMC may have potential as a novel antimetastasis agent for the treatment of human cervical cancer.