Interpreting the Collision Cross Sections of Native-like Protein Ions: Insights from Cation-to-Anion Proton-Transfer Reactions

摘要

The effects of charge state on structures of nativelike cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were probed using cation-to-anion proton transfer reactions (CAPTR), ion mobility, mass spectrometry, and complementary energy -dependent experiments. The CAPTR. products all have collision cross-section (52) values that are within 5.5% of the original precursor cations. The first CAPTR event for each precursor yields products that have smaller 52 values and frequently exhibit the greatest magnitude of change in 52 resulting from a single CAPTR event. To investigate how the structures of the precursors affect the structures of the products, ions were activated as a function of energy prior to CAPTR. In each case, the Omega values of the activated precursors increase with increasing energy, but the Omega values of the CAPTR products are smaller than the activated precursors. To investigate the stabilities of the CAPTR products, the products were activated immediately prior to ion mobility. These results show that additional structures with smaller or larger 52 values can be populated and that the structures and stabilities of these ions depend most strongly on the identity of the protein and the charge state of the product, rather than the charge state of the precursor or the number of CAPTR events. Together, these results indicate that the excess charges initially present on native-like ions have a modest, but sometimes statistically significant, effect on their 52 values. Therefore, potential contributions from charge state should be considered when using experimental Omega values to elucidate structures in solution.