Electrochemiluminescence Resonance Energy Transfer System for Dual-Wavelength Ratiometric miRNA Detection

摘要

In this study, we proposed a dual-wavelength electrochemilumi-nescence resonance energy transfer (ECL-RET) ratiometric sensor combined with duplex-specific nuclease (DSN)-assisted target recycling amplification to detect microRNAs (miRNAs). Due to the perfect overlapping of spectra, the gold-nanoparticle-luminol-layered-double-hydroxides (Au NP-luminol-LDH) nanocomposite and gold nanoclusters (Au NCs) exhibited excellent ECL-RET effect with high efficiency. The Au NP-luminol-LDH donor exhibits a strong and stable ECL emission at the wavelength peak of 440 nm, while the Au NC acceptor has an emission peak at 620 nm. Upon the introduction of DSN ltrigger the detachment of capture Au NCs-DNA from the surface of Au NP-uminol-LDH, resulting in an increased ECL signal of Au NP-Iuminol-LDH and and target miRNAs, the specific DNA-RNA binding and nuclease cleaving could a decreased fluorescence signal of Au NCs. By measuring the ratio of optical signals at 440 and 620 nm, the designed sensor provided a quantitative readout proportional to the target miRNAs concentration in the range of 10 aM to 100 pM with a lower detection limit (LOD) of 9.4 aM.