Single-Template Molecularly Imprinted Chiral Sensor for Simultaneous Recognition of Alanine and Tyrosine Enantiomers

摘要

Chiral recognition of L-amino acids is of significant importance due to the crucial role of L-amino acids in life sciences and pharmaceutics. In this work, a chiral sensor with capability of probing two chiral amino acids by an attractive single-template molecular imprinting strategy is introduced and used in the simultaneous chiral recognition of D/L-alanine (D/L-Ala) and D/L-tyrosine (D/L-Tyr). The assay relies on the hydrolysis of L-alanyl-L-tyrosine dipeptide doped in silica/polypyrrole (SiO2/PPy) under acidic conditions, resulting in L-Ala and L-Tyr coimprinted chiral sensor. This work opens up a new avenue for simultaneous chiral sensing of two or more chiral amino acids by incorporating only one template, circumventing the shortcomings encountered with multi-template molecularly imprinted technology.