The activation of N-methyl-D-aspartate receptors downregulates transient outward potassium and L-type calcium currents in rat models of depression

摘要

Major depression is an important clinical factor in ventricular arrhythmia. Patients diagnosed with major depression overexpress A'-methyl-D-aspartate receptors (NMDARs). Previous studies found that chronic NMDAR activation increases susceptibility to ventricular arrhythmias. We aimed to explore the mechanisms by which NMDAR activation may increase susceptibility to ventricular arrhythmias. Male rats were randomly assigned to either normal environments as control (CTL) group or 4 wk of chronic mild stress (CMS) to produce a major depression disorder (MDD) model group. After 4 wk of CMS, depression-like behaviors were measured in both groups. Varying doses (1-100μM) of NMDA and 10 μM NMDA antagonist (MK-801) were perfused through ventricular myocytes isolated from MDD rats to measure the L-type calcium current (I_(Ca-L)) and transient outward potassium current (I_(to)). Structural remodeling was assessed using serial histopathology including Masson's trichrome dye. Elec-trophysiological characteristics were evaluated using Langendorff perfusion. Depression-like behaviors were observed in MDD rats. MDD rats showed longer action potential durations at 90% repolar-ization and higher susceptibility to ventricular arrhythmias than CTL rats. MDD rats showed lower I_(Ca-L) and I_(to) current densities than CTL rats. Additionally, NMDA reduced both currents in a concentration-dependent manner, whereas there was no significant impact on the currents when perfused with MK-801. MDD rats exhibited significantly more fibrosis areas in heart tissue and reduced expression of Kv4.2, Kv4.3, and Cavl.2. We observed that acute NMDAR activation led to downregulation of potassium and L-type calcium currents in a rat model of depression, which may be the mechanism underlying ventricular arrhythmia promotion by depression.