Bile acids stimulate chloride secretion through CFTR and calcium-activated Cl~ channels in Calu-3 airway epithelial cells

摘要

Bile acids resulting from the aspiration of gastroesophageal refluxate are often present in the lower airways of people with cystic fibrosis and other respiratory distress diseases. Surprisingly, there is little or no information on the modulation of airway epithelial ion transport by bile acids. The secretory effect of a variety of conjugated and unconjugated secondary bile acids was investigated in Calu-3 airway epithelial cells grown under an air-liquid interface and mounted in Ussing chambers. Elec-trogenic transepithelial ion transport was measured as short-circuit current (/sc). The taurine-conjugated secondary bile acid, taurodeoxy-cholic acid (TDCA), was found to be the most potent modulator of basal ion transport. Acute treatment (5 min) of Calu-3 cells with TDCA (25 fiM) on the basolateral side caused a stimulation of /sc, and removal of extracellular Cl~ abolished this response. TDCA produced an increase in the cystic fibrosis transmembrane conductance regulator (CFTR)-dependent current that was abolished by pretreatment with the CFTR inhibitor CFTRinhi72- TDCA treatment also increased Cl~ secretion through calcium-activated chloride (CaCC) channels and increased the Na+/K+ pump current. Acute treatment with TDCA resulted in a rapid cellular influx of Ca2+ and increased cAMP levels in Calu-3 cells. Bile acid receptor-selective activation with INT-777 revealed TGR5 localized at the basolateral membrane as the receptor involved in TDCA-induced Cl~ secretion. In summary, we demonstrate for the first time that low concentrations of bile acids can modulate Cl~ secretion in airway epithelial cells, and this effect is dependent on both the duration and sidedness of exposure to the bile acid.