Flame-Spheroidized Phosphate-Based Glass Particles with Improved Characteristics for Applications in Mesenchymal Stem Cell Culture Therapy and Tissue Engineering

摘要

The chemical formulation of phosphate-based glasses (PBGs) can be tailored to fit particular end applications such as bone tissue engineering. While most reports to date have evaluated the effect of PBG chemical formulation on bone cells, this study specifically explored the manufacturing process, the changes in physical and chemical properties of PBG particles after flame spheroidization, and subsequent effects on human mesenchymal stem cells (hMSCs), a prime cell type for regenerative medicine applications. Flame spheroidization involves feeding irregular PBG particles (microparticles, MP) into a hot flame, causing them to melt and mold into solid spherical PBG particles (microspheres, MS). The laser diffraction analysis showed an increase in the volume-weighted mean diameter of particles from 48 to 139 ym after spheroidization and also revealed changes in the chemical composition of smaller MS ( 45 mu m in size), whereas MS in other size ranges did not show significantly different chemical composition compared to MP. Additionally, some air bubbles were entrapped inside particles during spheroidization, causing a 2% drop in relative density of MS. However, the packing density of MS was 30% higher than that of MP. Culture of hMSCs on the particles showed significant improvement in cell spreading on MS compared to that on MP and nearly 2 times higher cell metabolic activity after 7 days of culture, suggesting that MS provided a more favorable support and geometry for hMSC attachment and growth for tissue engineering.