Stanozolol promotes lipid deposition in the aorta through an imbalance in inflammatory cytokines and oxidative status in LDL LDL r knockout knockout mice fed a normal diet

摘要

Abstract The aim of the study was to evaluate the effect of an anabolic steroid, stanozolol, in a model of atherosclerosis and to investigate the involvement of the modulation of the inflammatory cytokines and oxidative stress in vascular lipid deposition. Low‐density lipid receptor‐deficient ( LDL r?/?) mice were fed a standard chow diet and were each week injected subcutaneously either saline (control C group) or 20?mg/kg stanozolol (S group). After 8?weeks, the levels of cholesterol, oxidized LDL (Ox LDL ) and cytokines were measured in plasma, lipid deposition in aorta was evaluated by en face analysis, and thiobarbituric acid‐reactive substances and oxidation protein were determined in liver. The S group demonstrated increases in vascular lipid deposition, triglycerides and non‐ HDL cholesterol levels. Stanozolol increased tumour necrosis factor alpha ( TNF ‐α) and decreased interleukin‐10 as well as increased the TNF ‐α/ IL ‐10 ratio. Furthermore, oxidative stress was observed in the S group, as indicated by an increase in the plasma Ox LDL , as well as by lipid peroxidation and oxidation of proteins in the liver. Chronic treatment with stanozolol promoted lipid deposition in the LDL r ?/? mice that could be attributed to a modification of the circulating cytokine levels and systemic oxidative stress. Our results suggest that the anabolic steroid stanozolol in the absence of functional LDL receptors by increasing systemic inflammation and oxidative stress may increase the risk of development and progression of atherosclerosis.