Structural characterization and evaluation of antibacterial and angiogenic potential of gallium-containing melt-derived and gel-derived glasses from CaO-SiO2 system

摘要

Gallium-containing glasses were synthesized by the sol-gel and traditional melting techniques to obtain bioactive amorphous materials with antibacterial and angiogenic properties. The influence of gallium ion addition on glass structure was described using two spectroscopic methods (FTIR and Si-29 MAS NMR). DSC (Differential Scanning Calorimetry) analysis revealed differences in thermal properties, which were explained by varying ionicity of the chemical bonds. The study shows that gallium ions addition causes a significant difference in glass structure and, as a consequence, in thermal properties, microstructure, and biological response. Dissolution products of the researched glasses incubated in SBF and distillated water were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). To perform the indirect in vitro biocompatibility studies, human osteoblast-like cells (MG-63) were cultivated in the supernatants, which were obtained by incubation of the glass powders in the cell culture medium. The evaluation of the potential angiogenic properties of gallium-doped glasses was performed by measuring the release of human vascular endothelial growth factor (VEGF) from MG-63 cells. Dissolution products of the all samples were tested for antibacterial activity against gram-negative (E. coli) and gram-positive bacteria (S. aureus).