Interactions between Sulfated Polysaccharides from Sea Brown Algae and Toll-Like Receptors on HEK293 Eukaryotic Cells In Vitro

摘要

We studied the interactions between sulfated polysaccharides, fucoidans from sea brown algae Laminaria japonica, Laminaria cichorioides, and Fucus evanescens, with human Tolllike receptors (TLR) expressed on membranes of cultured human embryonic kidney cells (HEK293-null, HEK293-TLR2/CD14, HEK293-hTLR4/CD14-MD2, and HEK293-hTLR5). Fucoidans interacted with TLR-2 and TLR-4, but not with TLR-5, and were nontoxic for the cell cultures. L. japonica fucoidan (1 mg/ml), L. cichorioides fucoidan (100 mu g/ml and 1 mg/ml), and F. evanescens fucoidan (10 mu g/ml-1 mg/ml) activated transcription nuclear factor NF-kappa B by binding specifi cally to TLR-2. L. japonica fucoidan (100 mu g/ml and 1 mg/ml), L. cichorioides fucoidan (10 mu g/ml-1 mg/ml), and F. evanescens fucoidan (1 mu g/ml-1 mg/ml) activated NF-kappa B via binding to TLR-4. These results indicated that fucoidans could induce in vivo defense from pathogenic microorganisms of various classes.