The lateral intercalated cell mass of the amygdala is activated during social buffering of conditioned fear responses in male rats

摘要

The presence of an affiliative conspecific reduces stress responses to a wide variety of stimuli. This phenomenon is termed "social buffering". We previously found that the presence of another Wistar rat (associate) suppressed activation of the lateral amygdala (LA) and ameliorated stress responses to an auditory conditioned stimulus (CS) in a fear-conditioned Wistar subject rat. Subsequent analyses suggested that activation of the posterior complex of the anterior olfactory nucleus (AOP) is responsible for the suppression of the LA. However, it remains unclear how the AOP suppresses the LA. To clarify this issue, a fear-conditioned Wistar subject was exposed to the CS either alone or with a Wistar associate. We also prepared a fear-conditioned Wistar subject that was tested with a Fischer344 (F344) associate as an additional control because F344 associates do not induce social buffering. We found that the presence of a Wistar associate induced a reduction of behavioral responses and Fos expression in the paraventricular nucleus of the hypothalamus (PVN) of the subject. Although Fos expression in the AOP was increased, the expression was not biased towards the GABAergic cells. In addition, Fos expression in the lateral intercalated cell mass of the amygdala (1ITC) was increased. In contrast, the presence of a F344 associate did not affect Fos expression in subjects' PVN or 1ITC, whereas behavioral responses were slightly reduced. These results suggest that the 1ITC was activated during social buffering. Based on these findings, we propose that the AOP indirectly suppresses the LA by activating the 1ITC.