A novel antimicrobial peptide isolated from centipede Scolopendra subspinipes mutilans stimulates neutrophil activity through formyl peptide receptor 2

摘要

Abstract In this study, we identified scolopendrasin X, a novel antimicrobial peptide (AMP), from centipede Scolopendra subspinipes mutilans . Scolopendrasin X strongly stimulated mouse neutrophils, resulting in intracellular calcium increase, chemotactic migration through pertussis toxin-sensitive G-protein and phospholipase C pathway, and increased superoxide anion production in neutrophils. Target receptor for scolopendrasin X, formyl peptide receptor (FPR)2 mediated scolopendrasin X-induced neutrophil activation. Moreover, scolopendrasin X significantly blocked inflammatory cytokine production induced by lipopolysaccharide in mouse neutrophils. Taken together, our results suggest that the novel AMP scolopendrasin X can be used as a material to regulate neutrophil activity through FPR2. Highlights ? A novel AMP scolopendrasin X stimulates calcium increase in neutrophils. ? Scolopendrasin X stimulates neutrophil chemotaxis via FPR2. ? Scolopendrasin X stimulates superoxide anion production in neutrophils. ? Scolopendrasin X blocks LPS-induced inflammatory cytokine production. ? A novel agonist for FPR2 regulates infectious/inflammatory responses.