The transcriptional factor YB-1 positively regulates Hsc70 transcription in Crassostrea hongkongensis

摘要

Abstract A Y-box binding protein Ch YB-1 was discovered as a ChHsc70 promoter-associated protein in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. The overexpression of Ch YB-1 in heterologous HEK293T cells led to clear enhancement of ChHsc70 promoter expression, while ChYB-1 depletion correlated with significant reduction of ChHsc70 transcription in the hemocytes of C.?hongkongensis . Quantitative Real-time PCR analysis revealed that both ChHsc70 and ChYB-1 were transcriptionally responsive to external chemical or physical stressors. This indicates a plausible correlation between ChHsc70 and ChYB-1 in the genetic regulatory pathway triggered by external stresses. This study presents the first evidence of positive regulator for Hsc70 transcription and contributes to a better understanding of the regulatory mechanisms governing Hsc70 expression. Highlights ? Ch YB-1 shows affinity to ChHsc70 promoter in Crassostrea hongkongensis. ? Ch YB-1 positively regulates ChHsc70 transcription. ? ChHsc70 and ChYB-1 transcriptions were coordinately induced by various stresses. ? ChYB-1 transcription tends to be more persistently induced by pollutants. ? This is the first report regarding positive regulator in controlling Hsc70 transcription.