Ethanol fermentation from non-detoxified lignocellulose hydrolysate by a multi-stress tolerant yeast Candida glycerinogenes mutant

摘要

The aim of this work was to study ethanol fermentation properties of the robust mutantCandida glycerinogenesUG21 from non-detoxified lignocellulose hydrolysate.C. glycerinogenesUG21 with high tolerance to elevated temperature, acetic acid, and furfural was obtained and applied in lignocellulose-based ethanol production.C. glycerinogenesUG21 exhibited highly-efficient degradation ability to furfural. High levels of acetic acid and furfural inhibited cell growths and ethanol production ofSaccharomyces cerevisiaeZWA46 and industrialAngelyeast but had a slight impact on biomass and ethanol titer ofC. glycerinogenesUG21. Using non-detoxified sugarcane bagasse hydrolysate,C. glycerinogenesUG21 reached 1.24?g/L/h of ethanol productivity at 40?°C but ethanol production ofS. cerevisiaeZWA46 andAngelyeast was inhibited. Further,C. glycerinogenesUG-21 exhibited 2.42-fold and 1.58-fold higher productivity thanS. cerevisiaeZWA46 andAngelyeast under low-toxicity hydrolysate. Therefore,C. glycerinogenesUG-21 could be an excellent candidate for low-cost lignocelluloses ethanol production.