One- and Two- Photon Auto-Fluorescence Micro-Spectroscopy of A Single Cell: Protoplast of Arabidopsis Thaliana And PC12 Cells

摘要

Fluorescence analysis is an important tool in life science research. The employment of laser in optical microscopy has greatly facilitated the advance in fluorescence excitation and analysis. In this article, we introduce the use of ultrafast laser in simultaneously forming optical trap and generating two-photon excitation. Specifically single cells prepared from the protoplast of Arabidopsis thaliana and PC 12 cell culture are used to demonstrate the application of auto-fluorescence in monitoring the cellular status.