A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system

Mu Di; Yan Liang; Tang Hui; Liao Yong

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A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system

机译：液滴数字聚合酶链反应扩增系统检测乙肝病毒共价闭合环状DNA的灵敏准确定量方法

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摘要

To develop a sensitive and accurate assay system for the quantification of covalently closed circular HBV DNA (cccDNA) for future clinical monitoring of cccDNA fluctuation during antiviral therapy in the liver of infected patients.

机译：开发一种灵敏而准确的测定系统，用于定量测定共价闭合的环状HBV DNA（cccDNA），以便将来在受感染患者肝脏中进行抗病毒治疗期间对cccDNA波动进行临床监测。

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《Biotechnology Letters》 |2015年第10期|共11页
作者
Mu Di; Yan Liang; Tang Hui; Liao Yong;
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正文语种 eng
中图分类分子生物学;
关键词
Covalently closed circular DNA (cccDNA); cccDNA-specific primer; Droplet digital PCR; Hepatitis B virus; HepG2.215 cell line; Plasmid-safe ATP-dependent deoxynuclease;

机译：共价闭合环状DNA（cccDNA）;cccDNA特异性引物;Droplet数字PCR;乙型肝炎病毒;HepG2.215细胞系;质粒安全的ATP依赖性脱氧核酸酶;

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专利

1. A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system [J] . Mu Di, Yan Liang, Tang Hui, Biotechnology Letters . 2015,第10期

机译：液滴数字聚合酶链反应扩增系统检测乙肝病毒共价闭合环状DNA的灵敏准确定量方法
2. Hepatitis B Virus Covalently Closed Circular DNA-Selective Droplet Digital PCR A Sensitive and Noninvasive Method for Hepatocellular Carcinoma Diagnosis? [J] . Shen Fan, Sergi Consolato, Sun Hui-lung The Journal of molecular diagnostics: JMD . 2018,第3期

机译：乙型肝炎病毒共价封闭圆形DNA - 选择性液滴数码PCR一种肝细胞癌诊断的敏感和非侵入性方法？
3. Nested real-time quantitative polymerase chain reaction assay for detection of hepatitis B virus covalently closed circular DNA [J] . XU Chun-hai, LI Zhao-shen, DAI Jun-ying, 中华医学杂志：英文版 . 2011,第010期

机译：巢式实时定量聚合酶链反应法检测乙型肝炎病毒共价闭合环状DNA
4. Design of Integrated Instrument for Polymerase Chain Reaction, Biochip Hybridization and Fluorescence Detection Part Two: A New Biochip to Detection Hepatitis B Virus Mutation [C] . Liu quanjun, Zhu Jijun, Zhao Wei, Instrumentation Science and Technology Vol.2 . 2002

机译：聚合酶链反应，生物芯片杂交和荧光检测集成仪器的设计第二部分：检测乙型肝炎病毒突变的新型生物芯片
5. Novel Methods for Specific Detection and Quantification of Covalently Closed Circular DNA in Sera and Biopsies of Hepatitis B Patients. [D] . Yu, Ling. 2011

机译：特异性检测和定量乙肝患者血清和活检中共价闭合环状DNA的新方法。
6. Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus [O] . Yuan Hui, Zhiming Wu, Zhiran Qin, 2018

机译：微滴数字聚合酶链反应和实时定量聚合酶链反应技术提供寨卡病毒的高灵敏度和准确检测
7. DNA Polymerase alpha is essential for intracellular amplification of hepatitis B virus covalently closed circular DNA [O] . Liudi Tang, Muhammad Sheraz, Michael McGrane, 2019

机译：DNA聚合酶α对于细胞内扩增对乙型肝炎病毒的细胞内扩增至关重要，共价封闭圆形DNA
8. Specificity of Polymerase Chain Amplification Reactions for Human Immunodeficiency Virus Type 1 DNA Sequences. (Reannouncement with New Availability Information). [R] . Bell, J., Ratner, L. 1989

机译：人类免疫缺陷病毒1型DNa序列的聚合酶链扩增反应的特异性。（重新公布新的可用性信息）。

A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system

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