The effect of fitness and varying location of the copia retrotransposon in Drosophila melanogaster inbred lines differing in transposition rate

摘要

Three sublines of an inbred laboratory line of Drosophila melanogaster with the initial copia transposition rate 2 x 10~-2, 2 x 10~-3, and 5 x 10~-4 per copy per generation were reared for several dozen generations under conditions of low effective population size (by full-sib crosses or in a small mass culture of 10 females x 10 males). All six lines were tested for the transposition rate, location pattern ,and copy number of copia in euchromatic genome regions and for fitness inferred from the intraspecific competition index. The copia transposition rate remained constant in both versions of the lines with an initially lower rate and decreased by an order of magnitude in both versions of the line with an initially higher rate. New copia insertions behaved as selectively neutral and were accumulated in the genome. Each new copy decreased fitness by less than 1% on average. Some of he existing unfixed insertions remained segregating after long-term inbreeding and were assumed to provide a selective advantage to heterozygotes.