Optimization of the medium and Conditions for Cultivation Erwinia sp., a Producer of Fumarase and Aspartase

摘要

Mathematical methods of experimental design were used to determine the optimal concenttrations of nutrient medium components, aeration conditions, and pH providing for the maximum biomass yields, as well as fumarase and aspartase activities, during submerged cultivation of Erwinia sp. The data showed that different concentrations of carbon source (molasses) and pH of the nutrient medium were required to reach the maximum yields of fumarase and aspartase. Calculations suggested that the combination of these optimized factors would result in 3.2-, 3.4- and 3.8-fold increases in the Erwinia sp. biomass, aspartase activity, and fumarase activity yields, respectively. The experimental data were consistent with these estimates to a 80% accuracy.