High-throughput Technology and High-throughput Factory

摘要

Structural Genomics has launched over the world including National Project of Protein Structural and Functional Analysis in Japan in a post-genomic era. It remains many bottlenecks to determine the protein structures with SR protein crystallography based on full-genome sequence information in high throughput manner. We review the bottlenecks and pit falls in the high throughput technologies from expression plasmid to protein structure in account of our experience of RIKEN Highthroughput Factory at Spring-8.