Effect of seed preconditioning and culture under different light intensities on callus induction and regeneration in excised cotyledon cultures of mungbean (Vigna radiata (L.) Wilczek).

摘要

V. radiata cv. PAEC5 seeds were soaked in sterile distilled water for (a) 16 or (b) 48 h, soaked in sterile distilled water for 16 h then placed on (c) plain agar, (d) Murashige and Skoog (MS) medium or (e) MS medium + benzyladenine (BA) for 48 h, or (f) placed on plain agar for 16 h. The cultures were incubated under full light (50 鎚ol m-2 s-1) or low light (8.5 鎚ol m-2 s-1). Cotyledons of the preconditioned seeds were excised and cultured on MS basal medium, MS medium + NAA + BA or B5 basal medium + NAA + BA. After 3 weeks of culture, (a), (b) and (c) had a lower percentage of callus formation than the other treatments, except in MS medium. Treatments (d), (e) and (f) had the highest callus growth ratings, particularly in the BA-supplemented media and under low light. Plantlet regeneration in B5 basal medium + NAA + BA was relatively higher than the other regeneration media under full light, while MS basal medium and MS basal medium + NAA + BA had higher plantlet regeneration under low light.