Electrophoretic study of polymorphism of grain storage proteins and histone H1 in Elytrigia repens (Poaceae).

摘要

Polymorphism of endosperm proteins and histone H1 in Eurasian biotypes of Elytrigia repens [Elymus repens] (genomic formula StStHH) have been studied by SDS-, AcOH and two-dimensional PAGE in comparison with StH-genomic Elymus species. The range ofmolecular weights (Mr) of the prolamine-glutelin complex polypeptides in E. repens seeds is 30-80 kD and does not differ from that in StH-genomic Elymus species. The strongly expressed prolamin component with Mr approximately 30 kD was found in all populations studied of E. repens covering an area from Western Europe up to Kamchatka. This component could be considered as species-specific. The high level of electrophoretic pattern variation of endosperm proteins and histone H1 (including sisterly individuals) has been shown. Nevertheless Elytrigia repens possesses a specific composition of histone H1 components which differs from that in StH-genomic Elymus species.