Modelling coupled dynamics of diffusive–convective mass transfer in a microfluidic device and determination of hydrodynamic dispersion coefficient

摘要

Highlights?The hydrodynamic dispersion coefficients were determined experimentally in PDMS microreactors.?A mathematical model was derived for the coupled dynamics of convective–diffusive mass transfer.?The relations between dispersion coefficients and flow rates were investigated.?The developed technique can be used to model different reactions catalysed by enzymes.AbstractOne of the challenges in mathematical modelling of microchips is the lack of available data for dispersion coefficients of biomolecules. The main focus of this study was to determine the hydrodnamic dispersion coefficients of the model substrates, 4-Nitrophenyl-β-d-glucopyranoside (pNPG_1) and 4-Nitrophenyl-β-d-glucuronide (pNPG_2) for β-glucosidase and β-glucoronidase. The substrate solutions were pumped through the sil