Whole Body Protein Turnover and Net Protein Balance After Pediatric Thoracic Surgery: A Noninvasive Single-Dose N-15 Glycine Stable Isotope Protocol With End-Product Enrichment

摘要

Background: We used the N-15 glycine urinary end-product enrichment technique to quantify whole body protein turnover following thoracic surgery. Materials and Methods: A single dose of N-15 glycine (2 mg/kg) was administered orally on postoperative day 1 to children (1-18 years) following thoracic surgery. N-15 enrichment of ammonia and urea was measured in mixed urine after 12 and 24 hours, respectively, and protein synthesis, breakdown, and net balance determined. Nitrogen balance (dietary intake minus urinary excretion) was calculated. Urinary 3-methylhistidine:creatinine ratio was measured as a marker of skeletal muscle protein breakdown. Results: We enrolled 19 subjectsmedian (interquartile range): age, 13.8 years (12.2-15.1); weight, 49.2 kg (38.4-60.8)who underwent thoracotomy (n = 12) or thoracoscopic (n = 7) surgery. Protein synthesis and breakdown by N-15 enrichment were 7.1 (5.5-9) and 7.1 (5.6-9) gkg(-1)d(-1) with ammonia (12 hours) as the end product, and 5.8 (3.8-6.7) and 6.7 (4.5-7.6) with urea (24 hours), respectively. Net protein balance by the N-15 glycine and urinary urea nitrogen methods were -0.34 (-0.47, -0.3) and -0.48 (-0.65, -0.28) gkg(-1)d(-1), respectively (r(s) = 0.828, P .001). Postoperative change in 3-methylhistidine:creatinine ratio did not correlate significantly with protein breakdown or balance. Conclusion: The single-dose oral administration of N-15 glycine stable isotope with measurement of urinary end-product enrichment is a feasible and noninvasive method to investigate whole body protein turnover in children. After major surgery, children manifest increased protein turnover and net negative balance due to increased protein breakdown.