首页> 外文期刊>Journal of the mechanical behavior of biomedical materials >Effect of epigallocatechin-3-gallate solutions on bond durability at the adhesive interface in caries-affected dentin
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Effect of epigallocatechin-3-gallate solutions on bond durability at the adhesive interface in caries-affected dentin

机译:EpigallocaTechin-3-gallate溶液对受龋齿粘连界面粘合耐久性的影响

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摘要

Hydrolytic and enzymatic degradation by matrix metalloproteinases (MMPs) reduces the durability of composite resin restorations on caries-affected dentin (CAD). The use of MMP inhibitors such as epigallocatechin-3-gallate (EGCG) could increase the longevity of the bond to dentin. This study aimed to evaluate the use of EGCG at different aqueous concentrations on the resin-dentin microtensile bond strength (mu TBS), fracture pattern and nanoleakage (NL) in immediate (IM) time interval and after 12-months of water storage (1Y) when using a two-step etch-and-rinse adhesive system on CAD. Dentin surfaces of 40 human molars were submitted to a microbiological caries induction protocol and randomized into 5 groups (n = 8) (0.02% EGCG; 0.2% EGCG; 0.5% EGCG; 2% Chlorhexidine [CHX] and no treatment as Control Group - [NT]). After acid etching, the solutions were applied for 60 s followed by application of dental adhesive (Adper Single Bond 2, 3 M ESPE) to CAD surfaces. Subsequently, a resin composite (4 mm) block was built on the dentin. After 24 h, the teeth were sectioned into beam-shaped specimens (cross-sectional area of 1 mm(2) and 8-mm high). Half of the specimens were tested in IM and the other half after 1Y. Two samples per tooth were submitted to SEM for NL evaluation. Data were statistically analyzed by two-way ANOVA and Tukey tests (alpha = 0.05). The results showed that use of EGCG and CHX did not affect mu TBS in IM (p 0.05). After 1Y, there was a reduction in mu TBS for all experimental groups (p 0.05). Adhesive fractures predominated in IM in all groups, except for 0.05% EGCG and NT. After 1Y, there was an increase in these adhesive fractures in all groups. For NL, all agents applied reduced NL in comparison with CT (p 0.001). CHX showed lower NL (p 0.001), followed by 0.02% and 0.5% EGCG. NT showed highest NL for both time intervals (p 0.001). Thus, although the use of EGCG at different concentrations and CHX reduced the NL, they were unable to reduce degradation of mu TBS to CAD over time.
机译:通过基质金属蛋白酶(MMP)的水解和酶促降解降低龋齿受影响的牙本质(CAD)的复合树脂修复物的耐久性。 MMP抑制剂如EPIGALLOCATECHIN-3-gallate(EGCG)可以增加债券的寿命。本研究旨在评估在急性浓度的不同含水浓度​​下在立即(IM)时间间隔内和储水12个月后(1Y) )在CAD上使用两步蚀刻和冲洗粘合剂系统。将40个人臼齿的牙本质表面提交至微生物龋齿诱导方案,并随机分为5组(n = 8)(0.02%EGCG; 0.2%EGCG; 0.5%EGCG; 2%氯己定[CHX]并没有治疗作为对照组 - [nt])。在酸蚀刻后,将溶液施加60秒,然后施加牙科粘合剂(Adper单键2,3m ESPE)至CAD表面。随后,在牙本质上建造了树脂复合物(4mm)块。 24小时后,将牙齿分成束形样品(横截面积为1mm(2)和8毫米)。在1Y之后,在IM和另一半的情况下测试了一半的标本。每个牙齿的两个样品被提交给SEM进行NL评估。通过双向ANOVA和TUKEY测试进行统计分析数据(alpha = 0.05)。结果表明,EGCG和CHX的使用不影响IM中的MU TBS(P&GT; 0.05)。 1Y之后,所有实验组的MU TBS还减少(P <0.05)。除了0.05%EGCG和NT之外,在所有组中占据在IM中的粘合剂骨折。 1Y之后,所有组中这些粘合剂骨折有增加。对于NL,与CT(P <0.001)相比,所有试剂施加了降低的NL。 CHX显示下部的NL(P <0.001),其次是0.02%和0.5%EGCG。对于两个时间间隔显示出最高NL(P <0.001)。因此,尽管在不同浓度和CHX下使用EGCG降低了NL,但它们不能随着时间的推移减少MU TBS的降解。

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