Waste derived sustainable carbon nanodots as a new approach for sensitive quantification of ethionamide and cell imaging

摘要

The quantification of Ethionamide (ETA) is of great importance due to their anti-tuberculosis activity and hazardous effect on humankind. Therefore, highly sensitive, selective simple and facile method for their detection will be highly appreciable. Herein we have attempted Carbon dot (C-CDs), synthesized from tender coconut waste, copiously available, by a simple hydrothermal treatment, as a fluorescent probe for the detection of ETA. The quenching of fluorescence of C-CDs with different concentrations of ETA is observed and this is due to the inner filter effect since absorption band of ETA overlaps with excitation and or emission of CDs. Under the optimized condition, a good linear quenching relationship is established in the range of 0-2.4 mu g mL(-1) with a limit of detection 0.33 mu g mL(-1). This proposed low-cost nanosensor system offers several advantages like simplicity, high selectivity, sensitivity, and rapid response. Hence, C-CDs based probe ultimately found excellent application for the detection of ETA in the urine and water samples giving a precise and highly reproducible result for these complex samples. Furthermore, C-CDs were anticipated for biological applications. The results revealed that C-CDs have less toxicity to the bacterial cells suggested excellent biocompatibility and ideal material for differentially staining of a yeast cell.