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Nitrogen doped waste tea residue derived carbon dots for selective quantification of tetracycline in urine and pharmaceutical samples and yeast cell imaging application

机译:氮掺杂废茶渣衍生的碳点用于尿液和药物样品中四环素的选择性定量和酵母细胞成像应用

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摘要

In this work, waste tea residue, abundantly accessible, is utilized for synthesis of fluorescent carbon dots (CDs) by a simple, low cost and one step chemical oxidation method. The optical properties, surface functionality and particle size of CDs were ascertained by various techniques. The CDs exhibited excellent water solubility, optical properties, bioimaging ability etc. The fluorescence intensity of the CDs was diminished gradually with increasing concentrations of tetracycline drug owing to the inner filter effect (IFE) phenomenon. The developed fluorescent probe displayed a better linear range, excellent detection limit and interference free response to the tetracycline. This resulted in quantification of tetracycline in real sample which achieved almost 99% recoveries indicated excellent practical potential. Moreover, the CDs were used as fluorescent label for multicolour imaging of yeast cells which could open the doors for further research in biological fields.
机译:在这项工作中,大量可利用的废茶残渣通过一种简单,低成本和一步化学氧化方法被用于合成荧光碳点(CD)。 CD的光学性质,表面官能度和粒度通过各种技术确定。 CD表现出优异的水溶性,光学性能,生物成像能力等。由于四环素药物的浓度增加,由于内部过滤效应(IFE)现象,CD的荧光强度逐渐降低。开发的荧光探针显示出更好的线性范围,出色的检测限和对四环素的无干扰响应。这样就可以对实际样品中的四环素进行定量,回收率达到近99%,表明具有极好的实用潜力。此外,CD还用作酵母细胞多色成像的荧光标记,这可能为生物领域的进一步研究打开大门。

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