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首页> 外文期刊>Journal of separation science. >Response surface methodology based on central composite design accompanied by multivariate curve resolution to model gradient hydrophilic interaction liquid chromatography: Prediction of separation for five major opium alkaloids
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Response surface methodology based on central composite design accompanied by multivariate curve resolution to model gradient hydrophilic interaction liquid chromatography: Prediction of separation for five major opium alkaloids

机译：基于中央复合设计的响应面方法伴随着模型梯度亲水性相互作用液相色谱的多变量曲线分辨率：五种主要罂粟的分离预测

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Abstract >Hydrophilic interaction liquid chromatography on bare silica presents some benefits for analysis and purification of ionizable basic alkaloids. This mode was used to separate five major opium alkaloids: morphine, codeine, thebaine, papaverine, and noscapine. Central composite design based on response surface methodology was applied for experimental design, modeling, and optimization in a single‐step gradient method. The main effects and their interactions (initial percentage of modifier, changing range of modifier in run time, pH of buffer, and its concentration) were investigated in 30 experiments. Multivariate curve resolution‐alternating least squares, by resolving overlapped curves, helped in the accurate calculation of baseline resolution factors to be modeled and optimized more accurately. Then three crucial resolution factors besides elution time were modeled in quadratic and cubic equations and optimized. In addition to the four factors, five extra logarithmic, and nonlogarithmic factors extracted from the four factors to give nine factors overall were inspected on mechanism of retention. It was shown that a linear combination consist of four independence variables successfully describes morphinans retentivity in a single‐step gradient method. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”> <标题类型=“main”>摘要</标题> >亲水性相互作用液相色谱在裸二氧化硅上呈现出可电离碱性生物碱的分析和纯化的一些益处。这种模式用于分离五个主要罂粟类生物碱：吗啡，可待因，伯因，罂粟碱和鼻腔。基于响应面方法的中央复合设计应用于单步梯度法的实验设计，建模和优化。在30个实验中研究了主要效果及其相互作用（改性剂的初始百分比，改变的改性范围，缓冲液的pH和其浓度）。通过解析重叠曲线的多变量曲线分辨率 - 交流最小二乘有助于准确计算基线分辨率因子，以更准确地建模和优化。然后在替二次和立方方程中建模除了洗脱时间之外的三个关键分辨率因子并进行了优化。除了从四个因素提取的四个因素，五种额外的对数和非曝光因素因子，在保留的机制上检查了整体的四个因素。结果表明，线性组合由四个独立变量组成，成功地描述了单步梯度法中的Morphinans保持性。</ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第18期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Taheri Mohammadreza&option=202" target="_blank" rel="nofollow">Taheri Mohammadreza;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bagheri Mohsen&option=202" target="_blank" rel="nofollow">Bagheri Mohsen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Moazeni‐Pourasil Roudabeh Sadat&option=202" target="_blank" rel="nofollow">Moazeni‐Pourasil Roudabeh Sadat;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ghassempour Alireza&option=202" target="_blank" rel="nofollow">Ghassempour Alireza;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Medicinal Plants and Drugs Research InstituteShahid Beheshti UniversityTehran Iran;</p> <p>Medicinal Plants and Drugs Research InstituteShahid Beheshti UniversityTehran Iran;</p> <p>Department of Analytical Chemistry Faculty of ChemistryKharazmi UniversityTehran Iran;</p> <p>Medicinal Plants and Drugs Research InstituteShahid Beheshti UniversityTehran Iran;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=central composite design&option=203" rel="nofollow">central composite design;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hydrophilic interaction liquid chromatography&option=203" rel="nofollow">hydrophilic interaction liquid chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=multivariate curve resolution&option=203" rel="nofollow">multivariate curve resolution;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=opium alkaloids&option=203" rel="nofollow">opium alkaloids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=response surface methodology&option=203" rel="nofollow">response surface methodology;</a> </p> <div class="translation"> 机译：中央复合设计;亲水性相互作用液相色谱;多变量曲线分辨率;罂粟类生物碱;响应面方法; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025847037.html">Response surface methodology based on central composite design accompanied by multivariate curve resolution to model gradient hydrophilic interaction liquid chromatography: Prediction of separation for five major opium alkaloids</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Taheri Mohammadreza&option=202" 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